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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: J Biol Chem. 2007 Mar 9;282(18):13395–13401. doi: 10.1074/jbc.M700498200

Fig. 2.

Fig. 2

FRB Library Screen and Validation of FRB Mutants in S. cerevisiae.

A, The library of FRB mutants expressed in yeast was treated with 1 μM iRap for 16 h and assayed by flow cytometry for yeGFP expression. The population was enriched from 3% to 55% positives with one round of sorting.

B, Each FRB mutant was individually transformed into yeast, treated with 1 μM iRap for 16 h and assayed by flow cytometry for yeGFP expression. Data are displayed as fold increase in fluorescence level over background, which was normalized to one. Experiments were performed in duplicate and the average of both experiments is shown.