Skip to main content
. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: J Biol Chem. 2007 Mar 9;282(18):13395–13401. doi: 10.1074/jbc.M700498200

Fig. 3.

Fig. 3

Secreted Alkaline Phosphatase (SeAP) activity of the FRBs in COS-1 cells.

COS-1 cells were co-transfected with Gal4BD-FKBP, VP16AD-FRB and reporter constructs and treated with iRap from 0.5-500 nM or rapamycin from 0.05-50 nM. Cells were treated with the quenched fluorescent substrate methylumbelliferylphosphate and assayed for SeAP expression. Data are shown with open symbols for rapamycin samples and filled symbols for iRap samples. Data for wtFRB and representative mutants are presented as the average (± SEM) for an experiment performed in quadruplicate.