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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: J Biol Chem. 2007 Mar 9;282(18):13395–13401. doi: 10.1074/jbc.M700498200

Fig. 4.

Fig. 4

Characterization of FRB Mutants in Mammalian Cells.

A, NIH3T3 cells were transduced with YFP, wtFRB-YFP or mutant FRB-YFP constructs, treated with 1 μM iRap for 24 h and assayed for YFP fluorescence by flow cytometry. 293T cells were transiently transfected with a wtFRB-YFP construct and treated as above. Data are presented as the mean fluorescence intensity (MFI) for each FRB construct with error bars for triplicate measurements.

B, NIH3T3 cells stably expressing wtFRB-YFP were treated with concentrations of iRap from 10 nM to 10 μM and YFP expression was monitored by flow cytometry for 24 h. Data are presented as the mean fluorescence intensity relative to the overall highest value. Experiment was performed in duplicate.