Table 1. FRB Mutants Treated with iRap Exhibit a Range of EC50 values by the SeAP Assay.
Fluorescence data generated by the SeAP assay were fit and EC50 values derived from the curve fits. Data are presented as the mean value (±SEM) for quadruplicate measurements. Identities of mutants are listed in supplemental Table S1.
| FRB Mutant | EC50 (±SEM) nM |
|---|---|
| WT FRB | 20.5 (0.6) |
| FRB19 | 14.4 (0.4) |
| FRB22 | 18.4 (0.8) |
| FRB11 | 19.3 (1.1) |
| FRB10 | 20.4 (1.2) |
| FRB20 | 22.1 (2.0) |
| FRB14 | 23.0 (0.6) |
| FRB2 | 24.9 (2.1) |
| FRB21 | 26.0 (1.1) |
| FRB18 | 28.1 (2.0) |
| FRB1 | 29.7 (2.2) |
| FRB6 | 31.4 (1.1) |
| FRB17 | 32.6 (1.5) |
| FRB9 | 33.4 (2.1) |
| FRB4 | 39.0 (5.3) |
| FRB8 | 39.1 (2.3) |
| FRB5 | 41.7 (4.6) |
| FRB16 | 42.7 (9.2) |
| FRB13 | 45.1 (10.5) |
| FRB3 | 67.4 (10.0) |
| FRB7 | 88.8 (19.3) |
| FRB12 | 100 (55) |
| FRB15 | 250 (140) |