(A) Cells growing in rich medium have few small peroxisomes per cell and peroxins and PpAtg30 are either weakly, or not, expressed.
(B) During peroxisome proliferation, the expression of peroxins and PpAtg30 is induced. PpAtg30 localizes at the peroxisome membrane by interacting with PpPex3 and PpPex14.
(C) Pexophagy conditions activate an unknown kinase that phosphorylates peroxisome-associated PpAtg30. PpAtg30 is not required for the formation of the peroxisome-sequestering arms, but is needed for movement of PpAtg11 to the arms. PpAtg30 delivers peroxisomes to the autophagy machinery via interaction with PpAtg11 and PpAtg17.
(D) The peroxisome cluster tagged with phosphorylated PpAtg30 is fully surrounded by the vacuole, sequestering arms, and MIPA.