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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: Cytoskeleton (Hoboken). 2012 Jul 31;69(9):613–624. doi: 10.1002/cm.21049

Fig. 2. Localization of dynein in mutant strains of N. crassa.

Fig. 2

A: Hyphae from the edges of colonies of wildtype and Loa mutant strains were visualized at intermediate magnification to show the distance between dynein signal and the hyphal tip (arrowheads). Bars: 50 μm. B: Hyphae visualized using higher magnification show details of dynein localization. In wildtype strains dynein is accumulated at hyphal tips and localizes to comet tails (red arrows) and to spherical structures (green arrows). In the Loa mutant strains, dynein showed reduced accumulation to hyphal tips as well as enhanced localization to spherical structures. Left panels = transmitted light; right panels = mCherry fluorescence. Arrowheads indicate position of the hyphal tip. Bars: 10 μm. Enlarged views of the boxed regions are shown on the right. C: Fluorescence intensity profiles as a function of distance from the hyphal tips for wildtype (olive) and Loa strains (maroon). Data are shown as mean ± S.E. (n = 15 hyphal tips for each strain).