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. Author manuscript; available in PMC: 2014 Sep 28.
Published in final edited form as: Cancer Discov. 2013 May;3(5):512–519. doi: 10.1158/2159-8290.CD-12-0426

Figure 3. G34 induces a transcriptional program linked to forebrain development and self-renewal.

Figure 3

DLX6: (a) ChIP-Seq of H3K36me3 and RNA polymerase II binding for G34 mutant KNS42 (blue) and wild-type SF188 cells (grey) for the DLX6 locus, which also encompasses the transcripts DLX5, DLX6-AS1 and DLX6-AS2. (b) Validation of ChIP-Seq data by ChIP-qPCR using specific primers targeting DLX6. Blue bars: KNS42, grey: SF188. *** p<0.0001, t-test. (c) Boxplot of DLX6 expression in the integrated paediatric GBM samples stratified by H3F3A status. Blue box: G34, green: K27, grey: wild-type. *** p<0.001, ANOVA. (d) Top – immunohistochemistry for DLX6 protein in a G34 mutant paediatric GBM sample RMH2465. Bottom – Barplot of DLX6 expression in a paediatric GBM tissue microarray stratified by H3F3A status. Blue bars: G34, green: K27, grey: wild-type. ++ strong expression, + moderate expression, - negative. SOX2: (e) ChIP-Seq of H3K36me3 and RNA polymerase II binding for G34 mutant KNS42 (blue) and wild-type SF188 cells (grey) for the SOX2 locus, which also encompasses the SOX2-OT transcript. (f) Validation of ChIP-Seq data by ChIP-qPCR using specific primers targeting SOX2. Blue bars: KNS42, grey: SF188. *** p<0.0001, t-test. (g) Boxplot of SOX2 expression in the integrated paediatric GBM samples stratified by H3F3A status. Blue box: G34, green: K27, grey: wild-type. * p<0.05, ANOVA. (h) Top – immunohistochemistry for SOX2 protein in a G34 mutant paediatric GBM sample RMH2465. Bottom – Barplot of SOX2 expression in a paediatric GBM tissue microarray stratified by H3F3A status. Blue bars: G34, green: K27, grey: wild-type. ++ strong expression, + moderate expression, - negative.