. Author manuscript; available in PMC: 2013 Sep 5.

Published in final edited form as: J Mol Biol. 2008 Jun 5;381(1):13–23. doi: 10.1016/j.jmb.2008.05.078

Table 2.

7-Methylguanine excision activities for wild-type and mutants of AlkD

	k_cat (10⁻³ min⁻¹)^a	Relative activity	Rate enhancement^b
WT	171.8 ± 11.1	(1.0)	231.6
D113N	1.8 ± 0.5	0.01	2.5
R148A	1.8 ± 0.3	0.01	2.5

First-order single-turnover rate constants for 7mG excision from a 25mer oligonucleotide duplex containing a 7mG·C base pair. Values represent the averages and standard deviations from three experiments.

Rate enhancements are based on the fold increase above a non-enzymatic control (k_non = 0.7 × 10⁻³ min⁻¹).