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. 2013 Sep 5;8(9):e73780. doi: 10.1371/journal.pone.0073780

Figure 5. Balsamin inhibits HIV-1 at the viral proteins synthesis step.

Figure 5

A. Primary CD4+ T cells were infected with DNase-treated single-round HIV-1 virus at a moi of 1 and 0.2, in the absence or presence of indicated doses of balsamin. Treatment with 50 µM AZT served as a positive control of HIV-1 inhibition. Forty-eight hours post-infection, cell lysates were prepared and subjected to Western blot analysis for HIV-1 p24 capsid and α-actin (as a loading control). The section of this figure is representative of two independent experiments. B. In parallel, cell-free supernatants were harvested from these cells 48 h of post-infection and viral replication was assessed by RT assay. The value obtained in the absence of balsamin at moi 1 was set as 100%. Data represent ±SD and is representative of two independent experiments performed in duplicate.