Figure 6. Balsamin potently inhibits influenza virus replication.

A. A549 respiratory cells were infected with influenza virus PR8 strain cells at a moi of 1, in the absence or presence of indicated doses of balsamin, and then washed after overnight incubation. Twenty-four hours later, the impact of balsamin on influenza virus proteins accumulation in cells was monitored by preparing cell lysates and analyzing them by Western blotting for viral M1 matrix protein. Actin served as a loading control. The section of this figure is representative of two independent experiments. B. In parallel, the impact of balsamin on influenza virus replication was assessed by titrating the viral content of infected cells supernatant on MDCK cells. Data represent ±SD and representative of three independent experiments performed in duplicate.