Figure 7. Detection of rare complex stabilizing mutations using off-rate classification models.

(A) Ranked list of 31 stabilizing mutations (Δlog₁₀(k_off) <−1) in SKEMPI off-rate dataset. The list is ranked according to the number of off-rate prediction classification models that detect the mutation in question as stabilizing. Detections per model (B) are highlighted in white, and non-detections highlighted in black. The lower portion of (A) is dominated by single-point mutations to alanine residues, which suggests that the stabilizing effects of these mutations, as opposed to their more common neutralizing/destabilizing effects, are much harder to characterize.