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. 2013 Sep 5;8(9):e74646. doi: 10.1371/journal.pone.0074646

Figure 8. qRT-PCR validation of microarray analysis results.

Figure 8

Relative changes in transcript abundance after gravity stimulation compared to the vertical control were analyzed by the microarray assay, and relative changes in transcript abundance observed by qRT-PCR all utilized the non-reoriented control at the relevant time point as a reference. A. qRT-PCR analysis of OsCML27 (Os03g21380), OsCPD2 (Os11g04710), a transcript encoding a putative receptor protein kinase (Os04g45730), Aux2 (Os09g37500) and LGC1 (Os02g30780) expression. B. qRT-PCR analysis of OsIAA20 (Os06g07040), and coding genes for cytochrome P450 (Os02g12690), UDP-D-xylose (Os12g25690), auxin efflux carrier component (Os01g51780), α-glucosidase precursor (Os06g46284), β-fructofuranosidase 1 (Os02g01590). 0.5 h down, 0.5 h up, 6 h down and 6 h up represent for lower flank at 0.5 h, upper flank at 0.5 h, lower flank at 6 h and upper flank at 6 h, respectively. The relative mRNA abundance was calculated as the ratio between the control sample and gravistimulated sample at each time point. Values are means ± SD; n = 3; □ real-time PCR results; ▪ microarray data.