Table 2.

Circulating levels (internal dose) of BPA measured in serum from lactating dams and their nursing pups at PND10 following continuous exposure during gestation/lactation (GD9–PND10).

BPA dose (μg/kg BW/day)	Animals	Total BPA			Unconjugated BPA			T:U BPA ratio
		Incidence of detectable BPA	Mean (ng/mL)	Range (ng/mL)	Incidence of detectable BPA	Mean (ng/mL)	Range (ng/mL)
0	Dams	2/6a	0.35 ± 0.43	0–0.9b	0/6	< LOD	0.0–0.3b	NA
0	Pups	0/6	< LOD	0–0.1b	0/6	< LOD	NA	NA
250	Dams	6/6	16.50 ± 13.00*	6–38.8	6/6	1.25 ± 0.44*	0.9–2.1c	13:1
250	Pups	2/6a	0.38 ± 0.26*	0.2–0.8b	0/6	< LOD	0.0–0.1b	NA
Abbreviations: NA, not applicable; T:U, total BPA:unconjugated BPA. Values are expressed as mean ± SD; n = 6 animals/treatment group. aNumber of animals with BPA measurements at or above the LOD (0.3 ng/mL; the lowest amount of BPA that can be detected with a defined probability). bAll concentrations were at or below the LOQ (0.9 ng/mL; the lowest amount of BPA that can be quantitatively determined with suitable precision and accuracy). cOne or more concentrations were at or below the LOQ (0.9 ng/mL). *p < 0.05 compared with vehicle control by Student’s t-test.