Figure 3. Histone H3K9me3 modification in the disiRNA loci is dependent on DIM-2.

The distribution of the H3K9me3 modification in wild type, dim-2^KO and dim-5^KO mutants at the disi-47 locus was determined by ChIP using an H3K9me3 antibody. Relative enrichment of DNA was calculated by normalizing with the relative DNA binding levels of the dim-5^KO mutant. The insert panel shows H3K9me3 ChIP assay results at the ζ-η region in the indicated strains.