Figure 4. DNA methylation and disiRNA in disi-47 locus requires transcription.

(A) The disiRNA distribution at disi-47 locus in wild-type and wc-2^KO strain. Arrows indicate the transcription start sites. dLRE and pLRE are the two WC complex binding sites. The red bars indicate approximate primer set locations (see Table S1 for primer sequences). (B) RT-qPCR analyses of frq mRNA. (C) RT-qPCR analyses of the transcripts in the frq promoter region from a wild-type strain grown in constant light (LL) or constant darkness (DD) conditions and the wc-2^KO strain grown in LL. (D) MeDIP results of the dLRE region in the wild-type (LL and DD conditions) and the wc-2^KO strains (LL). In (B), (C) and (D), three independent repeats were performed. Values are mean ± s.d.