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. 2013 Sep 5;9(9):e1003750. doi: 10.1371/journal.pgen.1003750

Figure 3. 3C analysis demonstrates long-distance interactions between the TT>A enhancer and the TNFAIP3 gene region.

Figure 3

a. The track on the top is the location of the TNFAIP3 primers used to identify potential amplified interaction fragments tested by 3C. Primers 6–10, 16 and 24 produced signals and are marked with asterisks. The middle and bottom track shows the genomic region of TNFAIP3 with the location of the promoter CpG island and ENCODE defined transcription factor binding sites. b. Increased relative interaction frequencies (RCF) were detected in three regions of TNFAIP3, the promoter, intron 2 and the 3′ untranslated region. c. Stimulation of THP1 cells with LPS results in increased 3C interactions between the TT>A enhancer and the TNFAIP3 promoter along with a concomitant increase in A20 expression and IκBα phosphorylation. Shown is a representative blot from 3 independent experiments. Statistical differences were determined using Student's t-test.