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. 2013 Sep 5;8(9):e71935. doi: 10.1371/journal.pone.0071935

Figure 12. Immunofluorescent determination of microtubule dynamics in MDA-MB-231 cells.

Figure 12

Double immunoflourecence was done to determine the compounds' effect after 24 h on microtubule dynamics within MDA-MB-231 cells. Tyrosinated (dynamic) microtubules are visualized as red, whereas the detyrosinated microtubules are stained green. The vehicle-treated cells (A) demonstrated an intact dynamic microtubule structure. The colchicine-exposed positive control cells (B) showed complete microtubule depolymerisation with few detyrosinated microtubule fragments remaining. ESE-16 (C), ESE-15-one (D) and EMBS-treated cells (E) demonstrated detyrosinated microtubule fragments. All treated cell samples demonstrated a decrease in cell density with rounded, shrunken cells. Apoptotic bodies are evident in the ESE-16 treated cells (X63 oil objective).