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. 2013 Sep 5;8(9):e63332. doi: 10.1371/journal.pone.0063332

Figure 2. Chronic high glucose stimulation induces p25 expression in Min6 cells.

Figure 2

A Endogenous p25 expression induced by high glucose (25 mM) (panel 1, lanes 3 and 4.) B Autoradiograph of phosphorylated histone H1 in the glucose induced Min6 cells (panel 1, lanes 2 and 4). The same cell lysates were used for the kinase assay using Cdk5 immunoprecipitates. C Quantification of Cdk5 activities in the glucose induced Min6 cells. D Insulin secretion assay. The insulin secretion was significantly inhibited in the high chronic glucose treated cells compared to the low glucose treated cells (* P<0.01). Data represent mean ± SE of three experiments.