Table 2. Main effects* on the expression of six common reference genes.
Gene | Time Effect | Treatment Effect | Colony Effect | Correlation with IAPV titer |
Actin | F(3,181) = 2.5, P = 0.058 | F(2,181) = 8.5, P<0.001 | F(1,181) = 7.5, P<0.001 | ηp2 = 0.09, p<0.001 |
28S rRNA | F(3,181) = 3.0, P = 0.032 | F(2,181) = 5.7, P = 0.004 | F(1,181) = 0.07, P = 0.787 | ηp2 = 0.16, p<0.001 |
18S rRNA | F(3,181) = 9.1, P<0.001 | F(2,181) = 9.2, P<0.001 | F(1,181) = 3.5, P = 0.062 | ηp2 = 0.11, p<0.001 |
RPS5 | F(3,181) = 14.6, P<0.001 | F(2,181) = 16.0, P<0.001 | F(6,181) = 1.4, P = 0.243 | ηp2 = 0.17, p<0.001 |
mGST1 | F(3,181) = 10.1, P<0.001 | F(2,181) = 9.2, P<0.001 | F(1,181) = 0.02, P = 0.876 | ηp2 = 0.06, p = 0.001 |
Histone H2A | F(3,181) = 3.5, P = 0.016 | F(2,181) = 11.1, P<0.001 | F(1,181) = 3.3, P = 0.069 | ηp2 = 0.18, p<0.001 |
Significant effects in bold.