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. 2013 Sep 5;8(9):e75114. doi: 10.1371/journal.pone.0075114

Figure 5. P450cam oxidation activities of the PUPPET linker variants.

Figure 5

The activities of (A) PUPPET-Pn (n = 1–5) and (B) PUPPET-Gn (n = 1–6) were evaluated in reaction mixtures containing 18 nM enzyme. The activity of the previously reported PUPPET, containing a GGGGSLVPRGSGGGGS linker (indicated as “O”), and the activity of an equimolar mixture of PdR, PdX and P450cam (indicated as “R”), were also evaluated under the same reaction conditions. Error bars represent the standard error of three replicates; *P < 0.05, ***P < 0.001 (Student’s t-test).