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. 2013 Sep 5;8(9):e72994. doi: 10.1371/journal.pone.0072994

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© 2013 Gilbert et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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AF cells derived from non-degenerate (n = 4) and degenerate (n = 3) IVDs were treated+/−1.0 Hz CTS in serum-free media and total protein extracted at timepoints of up to 20 minutes. Mechanically stimulated and unstimulated A) non-degenerate and B) degenerate protein samples (5 µg/well) were separated using 10% SDS-PAGE and probed using primary antibodies against phosphorylated FAK. Blots were then stripped using a stripping buffer, re-blocked and probed using an antibody against total FAK protein. C) The density of bands was quantified using a Syngene imaging system and the ratio of phosphorylated: total FAK protein normalised to timepoint controls and plotted as % change. *denotes a significant change (p≤0.05) between treatment groups.