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. 2013 Sep 5;9(9):e1003769. doi: 10.1371/journal.pgen.1003769

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© 2013 Verma, Idnurm

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) SDS-PAGE gel for C. neoformans recombinant (His)₆-Bwc2. Left to right, lane 1 is the low molecular weight Bio-Rad protein ladder, lane 2, 3, 4 are purified recombinant Bwc2 eluted fractions. Lane 5 is crude unpurified protein extract. (B) Gel mobility shift assay for a fragment of the JEC21 UVE1 promoter with purified Bwc2. Left to right, lane 1 contains just the UVE1 promoter P₁ (P_UVE1), lane 2, 3 and 4 contains P₁+Bwc2 20 µg, 50 µg and 20 µg+Zn²⁺ respectively. Lane 5 is loaded with the non-specific radiolabeled probe P₂. Lane 6, 7 are P₁+Bwc2 20 µg and 50 µg. Lane 8, 9 are competition with the increasing amount of specific probe cold P₁ to negate any binding observed in lane 7. Lane 10 is Bwc2 and P₂ with Zn²⁺.