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. 2013 Sep 5;8(9):e72907. doi: 10.1371/journal.pone.0072907

Figure 1. Generation of an early-induction DM1 mouse model.

Figure 1

A. Whole mount in-situ hybridization of embryos at different time points showing onset of Dmpk expression (represented by the blue color) is visible only by the 10th day of development (E10). B. Schematic diagram representing the protocol used to generate and study the EDM1 mice and adult-onset mice. The green line represents administration of doxycycline. The red arrow represents induction of the transgene resulting in an increase in toxic RNA. Arrowheads with a P represent phenotypic analysis while the final arrowhead P&T represents phenotypic analysis and tissues collection. C. Induction of the transgene was measured in whole embryos by qRT-PCR at varying time points as indicated. Significant doxycycline mediated transgene induction starts at embryonic day 10, similar to results for endogenous Dmpk (see Figure 1A.). D. Graph of toxic RNA levels in skeletal muscle shows no significant difference between the EDM1 and adult-onset DM1 mice. Number of samples (n) provided on the graph, averages listed in the tables below the graphs, error bars are SEM. Two Sample T-Test was used to determine statistical significance.