Figure 8. The reduction in CHIKV copies/ml serum after injection with siRNA Chik-1 and Chik-5 in C57BL/6 mice infected with CHIKV.

C57BL/6 mice (n = 15) were infected with CHIKV i.v. (1×10⁶ PFU CHKV; 100 µl of 10⁷pfu/ml) and viral RNA copies were checked in serum and muscle tissues. After 72 h p.i. mice were inoculated i.v. with 1 mg/kg body weight nc siRNA (A and B), 1 mg/kg body weight Chik-1 (n = 15) (C and D), 1 mg/kg body weight Chik-5 (n = 15) (E and F) and Comb-siRNA (n = 15) 1 mg/kg body weight each (G and H) and viral RNA copies were checked in serum and muscle tissues at indicated time after injection. CHIKV E3 RNA was quantitated using real time RT-PCR. Values are given as LOG10 RNA copies/ml serum and LOG10 RNA copies/mg of tissue. Significance Dunnett's test: *p<0.05; **p<0.01.