Table 2.
Muscle lipid composition in cytosolic and membrane fractions
| Lipid | TAG (μmol/mg protein) | DAG (μmol/mg protein) | PL (μmol/mg protein) | FA (μmol/mg protein) | ||||
|---|---|---|---|---|---|---|---|---|
| NGT | Type 2 diabetes | NGT | Type 2 diabetes | NGT | Type 2 diabetes | NGT | Type 2 diabetes | |
| SFA | ||||||||
| Cyto | 4.91 ± 0.84 | 4.67 ± 2.91 | 14.17 ± 16.26 | 19.35 ± 11.05 | 4.68 ± 5.91 | 4.96 ± 3.35 | 0.56 ± 0.38 | 0.95 ± 0.81 |
| Mem | 272 ± 157 | 261 ± 169 | 55 ± 31 | 102 ± 51* | 250 ± 63 | 231 ± 45 | 19.86 ± 9.37 | 18.37 ± 5.26 |
| MUFA | ||||||||
| Cyto | 3.39 ± 1.78 | 4.54 ± 2.87 | 1.45 ± 0.96 | 2.29 ± 1.92 | 0.99 ± 1.18 | 1.12 ± 0.61 | 0.19 ± 0.16 | 0.31 ± 0.16 |
| Mem | 340 ± 270 | 284 ± 181 | 48 ± 20 | 52 ± 20 | 60 ± 16 | 74 ± 31 | 6.88 ± 4.65 | 5.83 ± 2.78 |
| PUFA | ||||||||
| Cyto | 1.04 ± 0.73 | 1.86 ± 1.80 | 2.94 ± 3.56 | 3.69 ± 3.23 | 2.72 ± 4.06 | 1.83 ± 1.04 | 0.52 ± 0.84 | 0.39 ± 0.37 |
| Mem | 112 ± 85 | 73 ± 42 | 19.50 ± 12.53 | 19.39 ± 9.41 | 305 ± 87 | 252 ± 61 | 11.98 ± 12.58 | 7.05 ± 4.28 |
| Trans | ||||||||
| Cyto | 0.23 ± 0.08 | 0.39 ± 0.46 | 0.98 ± 1.18 | 1.52 ± 1.86 | 0.17 ± 0.28 | 0.19 ± 0.25 | 0.16 ± 0.21 | 0.18 ± 0.25 |
| Mem | 11.67 ± 14.75 | 7.97 ± 3.21 | 1.44 ± 0.99 | 7.95 ± 6.39* | 4.56 ± 0.96 | 4.58 ± 2.01 | 0.72 ± 0.39 | 0.74 ± 1.16 |
| Total | ||||||||
| Cyto | 9.58 ± 2.27 | 11.47 ± 7.32 | 19.54 ± 19.79 | 26.84 ± 13.27 | 8.57 ± 11.29 | 8.10 ± 4.17 | 1.43 ± 1.14 | 1.84 ± 0.93 |
| Mem | 736 ± 522 | 627 ± 387 | 124 ± 40 | 182 ± 73* | 620 ± 163 | 562 ± 117 | 41 ± 26 | 30 ± 8 |
| Total | 745 ± 520 | 638 ± 388 | 142 ± 45 | 209 ± 77* | 628 ± 164 | 569 ± 116 | 42 ± 26 | 32 ± 8 |
Values are means ± SD
* p < 0.05 men with NGT vs type 2 diabetes, unpaired samples t test
Cyto, cytosolic fraction; FA, fatty acid; Mem, membrane fraction; MUFA, monounsaturated fatty acid; PL, phospholipids; PUFA, polyunsaturated fatty acid; SFA, saturated fatty acid; Trans, trans fatty acid