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. 2013 Sep 6;4:350. doi: 10.3389/fpls.2013.00350

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Copyright © 2013 Roschzttardtz, Conéjéro, Divol, Alcon, Verdeil, Curie and Mari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of Ferritin in Arabidopsis leaves. Sections of rosette leaves from 3 week-old WT (B,C,E,F) or triple mutant Atfer1,3,4 (A,D) plants irrigated with either water (A–C) or 2 mM Fe-EDDHA (D–F) were probed with an anti-ferritin antibody and revealed with a secondary anti-rabbit antibody coupled to the Alexa Fluor® 488 fluorophore. Sections were stained with DAPI to reveal cell nuclei. Ferritin localization appears in green, DAPI fluorescence in blue and chlorophyll auto-fluorescence in red. Scale bars: 10 μm.