Table 2.

Enzyme kinetics parameters determined in this project and contrasted with those of [23].

Reaction	Isoenzyme	Parameter	Value	SEM (%)	Ref. [23]	Unit
ADH	Adh1p	kcat	176	1.3		s−1
ADH	Adh1p	Kacald	0.462	5.3	1.11	mM
ADH	Adh5p	kcat	0	0		s−1
ENO	Eno1p	kcat	7.6	1.9		s−1
ENO	Eno1p	Kp2g	0.043	10	0.04	mM
ENO	Eno2p	kcat	19.9			s−1
ENO	Eno2p	Kp2g	0.104		0.04	mM
FBA	Fba1p	kcat	4.14	1.5		s−1
FBA	Fba1p	Kf16bp	0.451	5.3	0.3	mM
GPM	Gpm1p	kcat	400			s−1
GPM	Gpm1p	Kp2g	1.41		0.08	mM
HXK	Glk1p	kcat	0.0721			s−1
HXK	Glk1p	Kglc	0.0106		0.08	mM
HXK	Glk1p	Katp	0.865		0.15	mM
HXK	Hxk1p	kcat	10.2			s−1
HXK	Hxk1p	Kglc	0.15		0.08	mM
HXK	Hxk1p	Katp	0.293		0.15	mM
HXK	Hxk2p	kcat	63.1			s−1
HXK	Hxk2p	Kglc	0.2		0.08	mM
HXK	Hxk2p	Katp	0.195		0.15	mM
PDC	Pdc1p	kcat	12.1	3.5		s−1
PDC	Pdc1p	Kpyr	8.5	10	4.33	mM
PDC	Pdc5p	kcat	10.3	2.1		s−1
PDC	Pdc5p	Kpyr	7.08	5.7	4.33	mM
PDC	Pdc6p	kcat	9.21			s−1
PDC	Pdc6p	Kpyr	2.92		4.33	mM
PFK	Pfk1p:Pfk2p	kcat	210	1.8		s−1
PGI	Pgi1p	kcat	487	3.7		s−1
PGI	Pgi1p	Kg6p	1.03	19	1.4	mM
PGI	Pgi1p	Kgf6p	0.307	6.8	0.3	mM
PGK	Pgk1p	kcat	58.6			s−1
PGK	Pgk1p	Kp3g	4.58		0.53	mM
PGK	Pgk1p	Katp	1.99		0.3	mM
PGK	Pgk1p	nHadp	2
PYK	Cdc19p	kcat	20.1	2.9		s−1
PYK	Cdc19p	Kpep	0.281	12	0.14	mM
PYK	Cdc19p	Kadp	0.243	13	0.53	mM
PYK	Pyk2p	kcat	0	0		s−1
TDH	Tdh1p	kcat	19.1	1.5		s−1
TDH	Tdh1p	Kgap	0.495	7.5	0.21	mM
TDH	Tdh2p	kcat	8.63			s−1
TDH	Tdh2p	Kgap	0.77		0.21	mM
TDH	Tdh3p	kcat	18.2	1.8		s−1
TDH	Tdh3p	Kgap	0.423	9.2	0.21	mM
TDH	Tdh3p	Kbpg	0.909		0.0098	mM
TPI	Tpi1p	kcat	564	1.5		s−1
TPI	Tpi1p	Kdhap	6.45	5		mM
TPI	Tpi1p	Kgap	5.25	12		mM
TPI	Tpi1p	Kigap	35.1	3.1		mM