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1H-15N HSQC spectrum of AYEdes. The HSQC spectrum of ∼1 mM 15N-AYEdes in 50 mM potassium phosphate (pH 7.0), 100 mM KCl, recorded at 298 K and 750 MHz. Peaks are labeled with the one-letter amino acid code and sequence number, unlabeled peaks in the upper right corner of the spectrum correspond to side-chain NH2 from Gln and Asn residues.
