Figure 5.

Recapitulation of RosettaDesign stabilization with minimal mutations in AYE. RosettaDesign models of top-scoring AYE mutants (side-chains, yellow) in the context of their AYEwt structural amino acid neighbors (side-chains, CPK) with the AYEwt protein backbone represented in ribbons (olive) are shown in the central column. The corresponding views of the AYEwt (mutated side-chains, cyan) and AYEdes_VJQ chain B(mutated side-chains, green) crystal structures are shown in the left and right columns, respectively. The mutations are labeled above the corresponding illustration in the central column.