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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: Dev Neurosci. 2013 Feb 27;35(0):182–196. doi: 10.1159/000346682

Figure 5. Shift in regional ratios suggests migration of microglia and OPCs.

Figure 5

The ratio, Iba1 PTA in mid corpus callosum (Mid-CC)/Iba1 PTA supraventricular white matter (SVWM), was examined at 24h, 48h, and 16 days (P21) after ligation as a measure of migration (n = 6 per group at each age, A). At 24h after ligation, there was a highly significant bilateral increase in this ratio in ligated animals, but 48h after ligation this ratio was comparable in controls and ligated mice, suggesting accelerated microglial migration from SVWM towards the mid-CC in injured mice. A similar ratio was calculated using OPC count Mid-CC/SVWM (n = 6 per group at each age, B), which showed a significant bilateral increase only at 48h after ligation. Since the OPC count in Mid-CC was reduced at 24h after ligation but had normalized at 48h after ligation (see Figure 4), this increased ratio is likely reflecting migration of OPCs from the SVWM to Mid-CC.