Figure 5.

The 100 kb BAC 54 and BAC 52 Transgenes Do Not Affect Circadian Phenotype Wheel running activity records and period estimates of Clock/+ F1 progeny from different BAC transgenic lines are shown. Both transgenic and control animals were behaviorally tested using the experimental protocol described in Figure 4 and the text.

(A) Activity records of line TG55 Clock/+ progeny, in which the full-length BAC 54 was the transgenic construct. (tg) indicates transgenic animals’ records.

(B) Activity records of line TG80 Clock/+ progeny, in which the 100 kb BAC 54 fragment was the transgenic construct.

(C) Activity records of line TG121 Clock/+ progeny, in which the full-length BAC 52 was the transgenic construct.

(D) Histogram of period estimates for 46 F1 animals from lines TG55, TG80, and TG121. Individual data points are indicated by open circles.

Not all individual points can be seen due to overlapping values. Number of animals from each line tested is indicated on the bottom of the bar. Among the Clock/+ mice from these lines, significant effects of transgenic construct (2 DF, F = 9.60, p < 0.0005), transgene presence (1DF, F = 16.55, p < 0.0005), as well as construct by transgene interactions (2 DF, F = 10.8, p < 0.0001) were detected (GLM ANOVA). TG55 transgenic progeny had significantly shorter periods than all other groups as determined by Tukey’s post hoc tests (p < 0.05). There were no significant differences between transgenic and non-transgenic Clock/+ progeny in lines TG80 and TG121.