Table 1.

Metabolic parameters in WT and Clock mutant mice. Serum triglyceride, cholesterol, glucose, insulin and leptin concentrations were determined in 7–8 month old WT and Clock mutant mice fed a regular diet ad libitum (n=4–8 mice per group). For the measurement of glucose, insulin and leptin, blood was collected at 4-hour interval over a 24-hour time period via an indwelling catheter (40 µl per blood sample), and the data were pooled to provide an overall mean (± SEM) value. For triglyceride and cholesterol measurement, a single blood sample (160 µl) was collected at ZT 0.

Metabolic parameters	WT	Clock	P value
Triglyceride (mg/dl)	136 ± 8	164 ± 8	P < 0.05
Cholesterol (mg/dl)	141 ± 9	163 ± 6	P < 0.05
Glucose (mg/dl)	130 ± 5	161 ± 7	P < 0.01
Insulin (ng/ml)	1.7 ± 0.3	1.1 ± 0.1	N.S.
Leptin (ng/ml)	3.4 ± 0.4	4.6 ± 0.3	P < 0.05