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. Author manuscript; available in PMC: 2013 Sep 6.
Published in final edited form as: Mol Immunol. 2008 Jun 27;45(13):3567–3572. doi: 10.1016/j.molimm.2008.05.014

Figure 2.

Figure 2

Adhesion assay with 300.19-hPD-L1 cells shows specific binding to hPD-1 and hB7-1. Wells were coated with B7-1-Ig, PD-1-Ig, or hIgG1-Fc (negative control) and blocked. 300.19-hPD-L1 cells were labeled with BCECF, incubated with 40 µg/mL of the indicated mAbs, introduced into the wells, and fluorescence was measured before and after washing. Untransfected 300.19 cells showed less than 10% binding (not shown). Patterns of binding are represented by the shading pattern: mAb that block neither the PD-L1:B7-1 or PD-L1:PD-1 interaction (gray stipple), mAbs that block both PD-L1:B7-1 and PD-L1:PD-1 interactions (diagonal lines), mAbs that block primarily the PD-L1:PD-1 interaction but not the PD-L1:B7-1 interaction (solid gray), isotype control mAbs (white), and the negative control (black). Specific p-values (n=6) for each clone are: 130002 (PD-1: 0.622, B7-1: 0.11), 29E.11D12 (PD-1: < 2x10−16, B7-1: 0.001), 29E.2A3 (PD-1: < 2x10−16, B7-1: 0.0007), 29E.5A9 (PD-1: < 2x10−16, B7-1: 4.7x10−6), MIH1 (PD-1: < 2x10−16, B7-1: 6.3x10−5), MIH3 (PD-1: 5.1x10−16, B7-1: 0.12).