Figure 3.
Flow cytometric analysis of PD-L1 expression on CD28high and CD28low CD8 T cells from human peripheral blood, ex vivo and after 1–3 days of stimulation with anti-CD3. PD-L1 is induced on activated T cells. Events shown have been gated on live cells by forward and side scatter, then gated on CD8+ cells. CD45RO expression was gated into high and low subgroups. The quadrant gates shown were defined from unstained or control samples. These results were representative of three donors.