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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Cell Host Microbe. 2013 Aug 14;14(2):125–135. doi: 10.1016/j.chom.2013.06.008

Figure 7. Fbw7 knockdown is insufficient for MCV sT-induced transformation.

Figure 7

Rat-1 cells were stably transduced with two lentiviral shRNAs (sh Fbw7.1 and shFbw7.2) targeting the rat specific Fbw7. Empty vector lentivirus, sT.LSD91-95A or wild type MCV sT (sT.wt) were individually transduced into Fbw7 knockdown cells and subjected to transformation assays. Transformation-induced foci were stained with crystal violet (upper). Colonies formed in soft-agar (middle) were counted to quantitate transformation activity (bottom). Error bars represent SEM; n = 3. The SCFFbw7 targeting by Fbw7 knockdown is not sufficient for rodent cell transformation but decreases soft agar growth induced by MCV sT. Fbw7 knockdown level was documented in Figure S6.