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. 2013 Jul 26;288(36):25749–25759. doi: 10.1074/jbc.M113.470518

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — IsdI-heme reaction products with characteristics of the staphylobilins are produced from either the ascorbic acid or NWMN2274/NADPH reactions. Reaction products were separated from proteins and analyzed by HPLC, and the elution profiles at 465 nm are shown for the ascorbic acid (A) and NWMN2274/NADPH (B) reactions. In both cases there are two major peaks that absorb at 465 nm and are labeled 1 and 2. Optical spectra for peaks 1 (C) and peaks 2 (D) from ascorbic acid (blue lines) and NWMN2274/NADPH (red lines) reactions are shown with wavelengths of maximum absorbance shown for each spectra either above or below the lines.