FIGURE 5.

The effect of PhLP isoforms on the assembly of the Gβγ dimer in cell culture. A, HEK 293 cells were co-transfected with plasmids encoding the indicated epitope-tagged proteins and analyzed by pull-down with anti-HA-agarose after 48 h. B, representative Western blots showing the levels of PhLP-FLAG and ^Δ1–83PhLP-FLAG in the indicated cellular extracts. C, Western blots showing the amounts of Gγ₁-HA and co-precipitated Gβ₁-myc and endogenous Gβ_{HEK 293} subunits in the pull-downs. D, designated protein bands in C were quantified, and their values from lanes 3 and 4 were normalized to those in lane 2. Error bars, S.E. with n = 5 and p < 0.1 (*) as determined by t test.