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. 2013 Jul 25;288(36):25760–25768. doi: 10.1074/jbc.M113.486258

TABLE 2.

Proteins identified in the pull-downs by LC/MS/MS

Protein Symbol Spectral counts
Tg(+) Tg(−)
Phosducin-like protein Pdcl 167 11
T-complex protein 1 subunit α TCP-1α 459 121
T-complex protein 1 subunit β TCP-1β 594 191
T-complex protein 1 subunit γ TCP-1γ 393 106
T-complex protein 1 subunit δ TCP-1δ 430 94
T-complex protein 1 subunit ϵ TCP-1ϵ 361 134
T-complex protein 1 subunit ζ TCP-1ζ 451 133
T-complex protein 1 subunit η TCP-1η 554 85
T-complex protein 1 subunit θ TCP-1θ 530 148
Integrin β-1 Itgb1 87 29
Sorting nexin 12 Snx12 61 24
Paralemmin-1 Palm 54 25
Oxysterol-binding protein 2 Osbp2 46 7
DNA-directed RNA polymerase I subunit RPA1 Polr1a 43 0
KLRAQ motif-containing protein 1 Klraq1 38 12
Destrin Dstn 37 8
Peripheral plasma membrane protein CASK Cask 37 18
WD repeat-containing protein 82 Wdr82 33 15
Phosphomannomutase 1 Pmm1 32 7
Exocyst complex component 1 (SEC3) Exoc1 29 4
Fructose-bisphosphate aldolase C Aldoc 29 10
Mage-d2 Maged2 29 8
26 S proteasome non-ATPase regulatory subunit 6 Psmd6 27 8
Sorting nexin-1 Snx1 27 13
26 S proteasome non-ATPase regulatory subunit 11 Psmd11 26 10
Mitochondria fission 1 protein Fis1 25 7
Mediator of RNA polymerase II transcription subunit 27 Med27 23 0
Segment polarity protein disheveled homolog DVL-2 Dvl2 23 0
Conserved oligomeric Golgi complex subunit 8 Cog8 22 0
Guanine nucleotide-binding protein, subunit β-1a Gnb1 237 215
Guanine nucleotide-binding protein, subunit α-1a Gnat1 8 84
Guanine nucleotide-binding protein, subunit γ-T1a Gngt1 0 21
Tubulin α-1Aa Tuba1a 441 560
Tubulin β-5a Tubb5 505 624
Tubulin β-2Ca Tubb2c 102 124
Tubulin β-2Aa Tubb2a 72 60
Actin, cytoplasmic 1a Actb 85 67

a Selection criteria of spectral count >20 and Tg(+)/Tg(−) > 2 are not met.