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Error-prone aminoacylation of tRNAAla and tRNAPhe by catalytic domain of pneumococcal AlaRS. Aminoacylation time courses in the presence of 40 μm [14C]alanine, [14C]serine, or [14C]glycine for 310 nm pneumococcal AlaRS catalytic domain. A and B, wild type pneumococcal tRNAAla (A) or tRNAPhe (B) was used at a concentration of 7 μm. Data sets are the average of three independent experiments. Error bars indicate S.E.
