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The U4:C69 mismatch is an anti-determinant for PheRS editing of Ala-tRNAPhe. A–D, deacylation time courses for AlaRS-generated Ser-tRNAPhe wild type (A), Ser-tRNAPheU4G (B), Ala-tRNAPhe wild type (C), and Ala-tRNAPheU4G (D) in the presence of 0.6 μm active PheRS. Aminoacylation reactions were carried out using 0.5 μm AlaRS catalytic domain, 7 μm tRNA transcript, and 100 μm [3H]serine or [3H]alanine. Data sets are the average of three independent experiments. Error bars indicate S.E.
