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The effect of EF-Tu on Gly-tRNAAla misaminoacylation by AlaRS. Misaminoacylation time courses for generation of Gly-tRNAAla by 310 nm full-length pneumococcal AlaRS in the presence or absence of 3 μm activated pneumococcal EF-Tu are shown. The concentration of wild type pneumococcal tRNAAla used was 7 μm. The concentration of [14C]glycine used was 110 μm. Data sets are the average of three independent experiments. Error bars indicate S.E.
