TABLE 1.
Kinetic parameters for phenylalanylation of tRNAPhe wild type of U4G by PheRS
| Km | Vmax | kcat | kcat/Km | |
|---|---|---|---|---|
| μm | μm/min/mg | s−1 | ||
| Wild type tRNAPhe | 1.05 ± 0.19 | 0.15 ± 0.04 | 0.32 ± 0.09 | 0.30 |
| Mutant tRNAPheU4G | 1.09 ± 0.30 | 0.38 ± 0.06 | 0.78 ± 0.10 | 0.72 |
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