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Main products of ectoenzyme activities in A549 and U87 cells incubated with NAD+ or AMP
Intact U87 and A549 cells were incubated with 0.4 mm NAD+ or AMP. The generation of the indicated products was evaluated by HPLC analyses. ND, not detectable.
| ADPR (NAD+ substrate) | AMP (NAD+ substrate) | Adenosine (AMP substrate) | |
|---|---|---|---|
| nmol/min/mg protein | nmol/min/mg protein | nmol/min/mg protein | |
| A549 | 2.11 | ND | 5.76 |
| U87 | 0.07 | 0.22 | 1.51 |
