FIGURE 2.

Regulation of NDST1 by miR-23a. A, the single miR-23a-binding site present in the Ndst1 3′-UTR was cloned into a pMIR-REPORT vector. Luciferase activity was assayed after 48 h in cell lysates from HEK293 cells co-transfected with the miR-23a pMIR-REPORT vector and either negative control (Neg. control) dsRNA or pre-miR-23a. The luciferase signals were normalized to control groups. B, HUVECs were transfected with pre-miR-23a, and Ndst1 mRNA levels were determined by qRT-PCR. C, a PCR approach using miR-23a as a primer for the reverse transcription step (see Fig. 1E for comparison) was used to investigate the binding of miR-23a to the Ndst1 3′-UTR. miR-9 was included for comparison. D, effects of pre-miR-23a on N-deacetylase activity in HUVEC lysates. Error bars represent S.E. (n = 3).