FIGURE 5.

Overexpression of miR-24 or suppression of NDST1 leads to reduced levels of VEGFR2. A, Western blot analysis showed reduced total levels of VEGFR2 and reduced phosphorylation of the downstream targets ERK1/2 in HUVECs in response to increased miR-24. Total (T) as well as phosphorylated (P) VEGFR2, AKT, and ERK1/2 were detected using dual labeling with fluorescent secondary antibodies. ctrl, control. B and C, quantifications of total VEGFR2 (B) and phosphorylated VEGFR2 (C) normalized to total AKT. Neg. control, negative control. D, overexpression of miR-24 did not reduce the level of phosphorylation per VEGFR2 in response to VEGFA-165. E, Western blot analysis showed reduced levels of total VEGFR2 in response to siRNA-mediated suppression of NDST1 as compared with control. Error bars represent S.E. (n = 3 except where indicated n.d., not determined; n = 2).