TABLE 4.

IC₅₀ values of alkylated iminosugars toward the β-glucosidase activity measured in the absence or presence of CBE, derived from data presented in Fig. 7

β-Glucosidase activity was measured in membranes prepared from GBA2-overexpressing SH-SY5Y cells, mouse testis, and mouse brain. Best curve fit for enzyme activity data was dependent on the presence of CBE, and on the type of imino sugar.

	NB-DNJ			NB-DGJ		AMP-DNJ
	−CBEa		+CBEb	−CBEb	+CBE	−CBEb	+CBEb
	nm	μm	nm	μm	μm	nm	nm
SH-SY5Y-GBA2 line 17	5.2	189	62.5	6.4	3.9c
Testis	6.8	105	70.0	1.2	5.6b
Brain	6.7	5.2	102	1.5	4.7c	0.7	0.9

^a Two-sites curve fit.

^b Sigmoidal curve fit.

^c Bell-shaped curve fit.