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. 2013 Jul 24;288(36):26067–26077. doi: 10.1074/jbc.M113.486175

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — The effects of SMC2 on CTCF-mediated regulation of rRNA gene transcription. A, Western blot analysis of SMC2 levels in NEs from SMC2 shRNA or empty vector stably expressed Hela-S3 cells. Ctrl, control. B, RT-qPCR analysis of pre-rRNA level after SMC2 knockdown. Mean values of three independent experiments ± S.D. are included. **, p < 0.01. C, Western blot analysis of FLAG and SMC2 levels in NEs from HeLa-S3 cells with either pOZN-CTCF or pOZN overexpression. β-actin was used as a loading control. D, RT-qPCR analysis of SMC2 expressions in pOZN-CTCF- or pOZN-overexpressed HeLa-S3 cells after SMC2 knockdown. E, RT-qPCR analysis of CTCF expressions in pOZN-CTCF- or pOZN-overexpressed HeLa-S3 cells after SMC2 knockdown. F, RT-qPCR analysis of pre-rRNA precursor expressions in pOZN-CTCF- or pOZN-overexpressed HeLa-S3 cells after SMC2 knockdown. *, p < 0.05; **, p < 0.01.