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. 2013 Jul 23;288(36):26157–26166. doi: 10.1074/jbc.M113.502492

FIGURE 2.

FIGURE 2.

Comparison of the early neural differentiation of rabbit pluripotent stem cells. A, immunocytochemical analysis of neural marker proteins in differentiated neural stem cells (Nestin), neurons (TUJ1), and glial astrocytes (GFAP) derived from rabbit ES cells (rdES2-1, top panels) or early iPS cells (e-iPS-L1, middle panels; e-iPS-S1, bottom panels) in the presence of RA and SB431542. Because astroglia support neuronal migration, axonal guidance, and enable neural stem cells to differentiate into neurons, the immunoreactive pattern of GFAP resembled that of TUJ1. B, relative differentiation efficiency is shown (the absence of RA and SB431542 treatment is denoted “1”). Two rabbit ES cell lines and six e-iPS cell lines responded variably to RA and SB431542. The liver-derived e-iPS cell lines showed a worse capacity to differentiate than did the rdES2-1 cells. The data are shown as the mean ± S.D. (each rabbit ES cell line, n = 5; each rabbit e-iPS cell line, n = 3). *, p < 0.05. C, immunocytochemical analysis of neural marker proteins in RA- and SB431542-treated cells derived from l-iPS cells (liver-derived l-iPS-L1 cells, top panels; stomach-derived l-iPS-S1 cells, bottom panels). D, relative differentiation efficiency of the l-iPS cell lines was almost the same as that of the ES cells. Data are shown as the mean ± S.D. (each rabbit ES cell line, n = 5; each rabbit l-iPS cell line, n = 4). Scale bars, 100 μm.