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. 2013 Jul 23;288(36):26157–26166. doi: 10.1074/jbc.M113.502492

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Comparison of the in vitro differentiation to oligodendrocytes by rabbit pluripotent stem cells derived from the liver or stomach. A, scheme of the strategy for oligodendrocyte differentiation. The induction of oligodendrocytes was examined after an additional 20 days in culture in the presence of Noggin. B, immunocytochemical analysis of the marker proteins for early oligodendrocytes (O1) and mature oligodendrocytes (CNPase) expressed by rabbit ES cells (rdES2–1, top panels) and l-iPS cells (liver-derived l-iPS-L1 cells, middle panels; stomach-derived l-iPS-S1 cells, bottom panels). C, quantification of the neural differentiation index of the iPS cell lines for O1 and the merged signals for O1 and CNPase compared with those of the rdES cells. Data are shown as the mean ± S.D. (each rabbit ES cell line, n = 5; each rabbit iPS cell line, n = 3). *, p < 0.05. Scale bars, 100 μm.