. 2013 Jul 8;288(36):26201–26208. doi: 10.1074/jbc.M113.465963

TABLE 2.

Enzymological characterization of UDP-Gal, UDP-GalNAc, and compounds 1 and 2 with AAGlyB

Kinetics	UDP-Gal^a	UDP-GalNAc^a	1^a	2
K_m donor (μm)	0.7 ± 0.1^b	1.7 ± 0.2^b	<0.4^c	6.5 ± 0.5^d
	0.7 ± 0.06^c
K_m acceptor (μm)	21 ± 2^e	7.9 ± 0.6^e	211 ± 24^c	56 ± 3^d
k_cat (s⁻¹)	0.37 ± 0.06^b	0.88 ± 0.07^b	0.024 ± 0.001^c	0.006 ± 0.0008^d
	0.25 ± 0.01^c	0.89 ± 0.01^d
	0.67 ± 0.06^d
K_i (μm)	NA^f	NA^f	0.53^b^,^g	3.0^b^,^g
			0.52^b^,^h

^a Kinetic values, except for the CE k_cat values, were previously reported in Ref. 8.

^b Radiochemical assay, with 100 μm acceptor.

^c HPLC assay.

^d CE assay with tetramethylrhodamine-labeled HAA.

^e Radiochemical assay, with 100 μm donor.

^f NA, not applicable.

^g Donor: UDP-Gal (2 μm).

^h Donor: UDP-GalNAc (2 μm).